Identification of epitopes recognized by monoclonal antibodies directed against HTLV-I envelope surface glycoprotein using peptide phage display
Identifieur interne : 003393 ( Main/Exploration ); précédent : 003392; suivant : 003394Identification of epitopes recognized by monoclonal antibodies directed against HTLV-I envelope surface glycoprotein using peptide phage display
Auteurs : J. L. Chagnaud [France] ; D. Moynet [France] ; D. Londos-Gagliardi [France] ; J. H. Bezian [France] ; P. Vincendeau [France] ; H. Fleury [France] ; B. Guillemain [France]Source :
- Letters in Peptide Science [ 0929-5666 ] ; 2001-03-01.
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Abstract
Summary: Phage peptide libraries constitute powerful tools for the mapping of epitopes recognized by monoclonal antibodies (mAbs). Using screening of phage displayed random peptide libraries we have characterized the binding epitopes of three mAbs directed against the surface envelope glycoprotein (gp46) of the human T-cell leukemia virus type I (HTLV-I). Two phage libraries, displaying random heptapeptides with or without flanking cysteine residues, were screened for binding to mAbs 7G5D8, DB4 and 4F5F6. The SSSSTPL consensus sequence isolated from constrained heptapeptide library defines the epitope recognized by DB4 mAb and corresponds to the exact region 249–252 of the virus sequence. The APPMLPH consensus sequence isolated from non constrained heptapeptide library defines the epitope recognized by 7G5D8 mAb and corresponds to the region 187–193 with a single amino acid substitution, methionine to leucine at position 190. The third consensus sequence LYWPHD isolated from constrained heptapeptide library defines the epitope recognized by 4F5F6 mAb. It corresponds to an epitope without direct equivalence with the virus sequence. The data presented here showed that 7G5D8 and DB4 mAbs are raised against linear epitopes while 4F5F6 mAb recognized a continoous topographic epitope.
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DOI: 10.1007/BF02443610
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Using screening of phage displayed random peptide libraries we have characterized the binding epitopes of three mAbs directed against the surface envelope glycoprotein (gp46) of the human T-cell leukemia virus type I (HTLV-I). Two phage libraries, displaying random heptapeptides with or without flanking cysteine residues, were screened for binding to mAbs 7G5D8, DB4 and 4F5F6. The SSSSTPL consensus sequence isolated from constrained heptapeptide library defines the epitope recognized by DB4 mAb and corresponds to the exact region 249–252 of the virus sequence. The APPMLPH consensus sequence isolated from non constrained heptapeptide library defines the epitope recognized by 7G5D8 mAb and corresponds to the region 187–193 with a single amino acid substitution, methionine to leucine at position 190. The third consensus sequence LYWPHD isolated from constrained heptapeptide library defines the epitope recognized by 4F5F6 mAb. It corresponds to an epitope without direct equivalence with the virus sequence. The data presented here showed that 7G5D8 and DB4 mAbs are raised against linear epitopes while 4F5F6 mAb recognized a continoous topographic epitope.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Aquitaine</li><li>Nouvelle-Aquitaine</li></region><settlement><li>Bordeaux</li><li>Pessac</li></settlement></list><tree><country name="France"><region name="Nouvelle-Aquitaine"><name sortKey="Chagnaud, J L" sort="Chagnaud, J L" uniqKey="Chagnaud J" first="J. L." last="Chagnaud">J. L. Chagnaud</name></region><name sortKey="Bezian, J H" sort="Bezian, J H" uniqKey="Bezian J" first="J. H." last="Bezian">J. H. Bezian</name><name sortKey="Bezian, J H" sort="Bezian, J H" uniqKey="Bezian J" first="J. H." last="Bezian">J. H. Bezian</name><name sortKey="Chagnaud, J L" sort="Chagnaud, J L" uniqKey="Chagnaud J" first="J. L." last="Chagnaud">J. L. Chagnaud</name><name sortKey="Fleury, H" sort="Fleury, H" uniqKey="Fleury H" first="H." last="Fleury">H. Fleury</name><name sortKey="Guillemain, B" sort="Guillemain, B" uniqKey="Guillemain B" first="B." last="Guillemain">B. Guillemain</name><name sortKey="Guillemain, B" sort="Guillemain, B" uniqKey="Guillemain B" first="B." last="Guillemain">B. Guillemain</name><name sortKey="Londos Gagliardi, D" sort="Londos Gagliardi, D" uniqKey="Londos Gagliardi D" first="D." last="Londos-Gagliardi">D. Londos-Gagliardi</name><name sortKey="Moynet, D" sort="Moynet, D" uniqKey="Moynet D" first="D." last="Moynet">D. Moynet</name><name sortKey="Moynet, D" sort="Moynet, D" uniqKey="Moynet D" first="D." last="Moynet">D. Moynet</name><name sortKey="Moynet, D" sort="Moynet, D" uniqKey="Moynet D" first="D." last="Moynet">D. Moynet</name><name sortKey="Vincendeau, P" sort="Vincendeau, P" uniqKey="Vincendeau P" first="P." last="Vincendeau">P. 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